| Autor: |
Shepilov, D., Osadchenko, I., Ilienko, D., Chereshynska, A., Hryb, V., Kovalenko, T. |
| Předmět: |
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| Zdroj: |
Physiological Journal / Fiziologichnyi Zhurnal; 2022 Supplement, Vol. 68, p45-45, 1/2p |
| Abstrakt: |
Aim. The present study aimed to evaluate whether antibiotic treatment during gestation can trigger memory decline and brain structural alterations in mouse offspring. Methods. C57BL/6J female mice were divided into two groups (n=3/group): 1) Control -- drank sterile water over the entire gestation, and 2) Antibiotics -- consumed amoxicillin (205 mg/kg bw/day) and azithromycin (51 mg/kg bw/day) in sterile water during the 3rd week of gestation. Behavioral tests, immunohistochemistry of the hippocampus, and electron microscopy of the corpus callosum were conducted on 4-week-old mice born from those dams (n=13-16/group). Results. We revealed that, in Morris water maze, the Antibiotics group had significantly higher latency to find a hidden platform on the 2nd and 3rd days of acquisition and spent less time in the target quadrant during the probe test. T-maze spontaneous alternation test, in turn, did not show a significant difference in spatial working memory between groups. At the morphological level, mice from the Antibiotics group were characterized by a decrease in density of CA1 pyramidal neurons in the hippocampus, depletion of both DCX immunoreactivity and BrdU/DCX ratio in the dentate gyrus, as well as thinning of myelin sheaths and tendency to reduce the number of myelinated axons in the corpus callosum, compared to the control animals. Conclusions. Antibiotic treatment during gestation was demonstrated to impact the developing brain, resulting in spatial reference memory impairments, altered hippocampal cellular structure, reduced neurogenesis in the dentate gyrus, and signs of demyelination in young offspring mice. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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