| Autor: |
Varsaki, Athanasia, Ortiz, Sagrario, Santorum, Patricia, López, Pilar, López-Alonso, Victoria, Martínez-Suárez, Joaquín V. |
| Předmět: |
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| Zdroj: |
Zoonotic Diseases (2813-0227); Sep2022, Vol. 2 Issue 3, p82-94, 13p |
| Abstrakt: |
Simple Summary: In this study, the origin and persistence of Campylobacter jejuni was studied in dairy cattle farms in order to avoid recirculation of this leading cause of foodborne illness. A high level of genetic diversity and antimicrobial resistance was found, particularly in ciprofloxacin. As a result, the survival of antimicrobial resistant C. jejuni in cattle manure may pose a risk for human populations. The aim of this study was the examination of the genetic diversity and antimicrobial susceptibility of C. jejuni isolates obtained from dairy farms situated in Cantabria. The presence of Campylobacter jejuni was scrutinized in dairy farms situated in the Cantabria region (Atlantic coast, North of Spain). A total of 520 samples were collected from 12 dairy farms and 62 C. jejuni isolates were achieved. Sixty-one (61) of the isolates proceeded from fresh feces and only one from the stable (soil). Characterization of the isolates was conducted by Pulsed Field Gel Electrophoresis (PFGE) analysis. Antimicrobial susceptibility testing was carried out by standardized disk diffusion test. The PFGE analysis showed a high genetic diversity. From the 62 C. jejuni isolates, 27 different PFGE types were obtained with 70% similarity. The results of the antimicrobial susceptibility tests showed that 21 out of 27 strains were resistant to ciprofloxacin (78%), and 15 of them were also resistant to tetracycline (55%), whereas none of the 27 strains analyzed were resistant to erythromycin. C. jejuni was capable of surviving in livestock waste for at least 20–25 days, whereas the maximum detectable survival time on crops was of six days. This study reveals the high genetic diversity and ciprofloxacin resistance of C. jejuni in dairy cattle farms in Northern Spain, a fact that highlights the urgent need for the surveillance and control of this foodborne pathogen. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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