| Autor: |
Bajhan, Elshan, Mansoori, Behzad, Mohammadi, Ali, Shanehbandi, Dariush, Khaze Shahgoli, Vahid, Baghbani, Elham, Hajiasgharzadeh, Khalil, Baradaran, Behzad |
| Předmět: |
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| Zdroj: |
Archives of Physiology & Biochemistry; Oct2022, Vol. 128 Issue 5, p1323-1329, 7p |
| Abstrakt: |
Background: Prostate cancer (PC) is one of the most prevalent types of malignancies in males. Here, we replaced the miRNA-143 in PC cells by using a vector-based miRNA-143 transfection approach. Materials and methods: The miRNA-143 vector was transfected into the cells and qRT-PCR was applied to assess the expression of target genes in PC3 cells. Also, the MTT, scratch wound-healing, and DAPI staining assays were done to assess the proliferation, migration, and apoptosis of the cells, respectively. Results: The findings of the qRT-PCR determined the enhanced expression of miRNA-143 and other cancer-associated genes. The MTT and wound-healing assays revealed the proliferation and migration reduction in the transfected cells in comparison to control cells that contain an empty vector. Conclusion: The miRNA-143 has a significant impact on cell growth and migration during PC metastasis, and it may be a promising candidate for molecular therapies of PC. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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