| Autor: |
Rahimzadeh, Fatemeh, Ghadam, Parinaz, Kasra-Kermanshahi, Rouha, Zarrabi, Mahboobeh |
| Předmět: |
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| Zdroj: |
Polymer Bulletin; Oct2022, Vol. 79 Issue 10, p8353-8367, 15p |
| Abstrakt: |
One way to overcome the challenge of the active surface of nanoparticles and their tendency to aggregate is to use a biopolymer. The surface layer protein (S-layer) is a good candidate for trapping nanoparticles. S-layer molecules bridge the space between neighbor nanoparticles and prevent them from aggregating. In this study, S-layer as a renewable biopolymer was extracted from Lactobacillus helveticus, nanobiocomposite prepared by Intermatrix synthesis (IMS) method and S-layer used as a matrix for silver nanoparticles(AgNPs). The 3D structure of Lactobacillus helveticus S-layer was predicted with I-TASSER server, and then the binding of silver ions to this S-layer was confirmed by Molegro Virtual Docker software. After precursor ions binding to S-layer, the aqueous extract of dried Juglans regia green husk was added as a nanoparticle producing agent. Nanobiocomposite analysis with UV–Vis spectroscopy showed that the process was completed after 23 days. Then with the Field Emission Scanning Electron Microscope (FESEM), the distribution of nanoparticles on the S-layer matrix was observed. With the Energy-dispersive X-ray (EDX), the presence of AgNPs was confirmed. The antimicrobial effect of the nanobiocomposite against Pseudomonas aeruginosa PAO1 was investigated by the agar well diffusion method. There are three achievements in this study: (1) AgNPs were stabilized, (2) the antibacterial bionanocomposte was synthesized, and (3) renewable biopolymer (S-layer) as matrix and the aqueous extract of dried Juglans regia green husk as producing agent of AgNPs, introduce an ecofriendly method for fabrication of bionanocomposite. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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