| Abstrakt: |
The determination of biochemical and immunological indicators reflecting the state of regulatory systems of the organism involved in adaptation to physical activity was carried out. The study involved 10 figure skaters involved in pair skating. The average age was 16 ± 0.4 years; sports experience was 9 ± 1 years. The results of the biochemical analysis showed normal values for direct bilirubin, calcium, magnesium, and iron. In three athletes, a 1.5–2-fold decrease in glucose and an increase in creatinine levels were observed, which characterized the rate of energy accumulation and consumption depending on the functional state of the athlete. Consistency in the measurement of biochemical parameters was found in the process of dynamic observations against the background of heavy training loads. During the five study stages, there were no distinct shifts in biochemical parameters towards adaptation stress and deterioration of recovery over 62 days of athlete preparation for competitions. Unlike the generally accepted indicators of biochemical analysis, the results of immunoassay were informative and allowed us to determine the importance of endogenous regulators of adaptation to physical activity in the dynamics of training. The enzyme-linked immunosorbent assay (ELISA) was used to measure the level of natural antibodies to serotonin, histamine, dopamine, glutamate, GABA, orphanin, β-endorphin, and angiotensin in the blood serum of athletes. It was found that each athlete had an individual immune profile. The level of antibodies to β-endorphin was within the normal range, except for its decrease in two athletes. The level of antibodies to orphanin exceeded the normal value in the majority of subjects, which may be associated with the inhibitory control of pain signals. The indicators for antibodies to serotonin were above the normal range in 80% of cases and to dopamine, in 30% of athletes, suggesting the activation of the hypothalamic–pituitary–adrenal system (HPAS). The comparison of levels of antibodies to GABA and glutamate in individual samples showed that the normal range was exceeded for all subjects. The immunological indicators of antibodies to GABA and glutamate, orphanin, and serotonin were the most informative for establishing the "weak" links of adaptation. [ABSTRACT FROM AUTHOR] |
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