| Abstrakt: |
The article sheds light on the factors in the colorimetric estimation of 17-ketosteroids in urine. The purpose of this investigation has been two-fold, firstly, to examine some of the methods of 17-ketosteroid estimation. Secondly, to compare results obtained by the use of a correction formula for interfering chromogens with those of a simple procedure for 17-ketosteroid determination which obviates the need for such a formula. Using the technique described, the effect of acid hydrolysis on certain 17-ketosteroids has been investigated. Known amounts of both androsterone and dehydroisoandrosterone in ethanol were added to urine both before and after acid hydrolysis. |
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