| Autor: |
Zhu, Kevin W., Burton, Shawn D., Nagai, Maira H., Silverman, Justin D., de March, Claire A., Wachowiak, Matt, Matsunami, Hiroaki |
| Předmět: |
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| Zdroj: |
Nature Communications; 9/1/2022, Vol. 13 Issue 1, p1-18, 18p |
| Abstrakt: |
Sensory processing in olfactory systems is organized across olfactory bulb glomeruli, wherein axons of peripheral sensory neurons expressing the same olfactory receptor co-terminate to transmit receptor-specific activity to central neurons. Understanding how receptors map to glomeruli is therefore critical to understanding olfaction. High-throughput spatial transcriptomics is a rapidly advancing field, but low-abundance olfactory receptor expression within glomeruli has previously precluded high-throughput mapping of receptors to glomeruli in the mouse. Here we combined sequential sectioning along the anteroposterior, dorsoventral, and mediolateral axes with target capture enrichment sequencing to overcome low-abundance target expression. This strategy allowed us to spatially map 86% of olfactory receptors across the olfactory bulb and uncover a relationship between OR sequence and glomerular position. Targeted spatial transcriptomics mapped olfactory receptor mRNAs to sections of the murine olfactory bulb to generate an interactive, statistical, 3D model of glomeruli locations and identify an ultra-sensitive receptor-odorant relationship. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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