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S04-01 Regulation of microglia morphology and surveillance in health and disease Lorena Arancibia Carcamo I University College London, UK Dementia Research Institute, London, UK i Genetic studies have strongly implicated a role for microglia in the development of Alzheimer's disease, and transcriptomic studies have revealed profound changes in the cell states of microglia during disease progression. Although it is well established that neuron-derived fractalkine (CX3CL1) mediates the interactions between neurons and CX3CR1-expressing microglia, the number of microglia did not decrease in the brain of Cx3cr1-deficient mice. To dissect the regulatory mechanisms of SEMA6A/PLXN signaling between neurons and glia during cortical neuron migration, we devised an assay to target radial glial cells by ex vivo electroporation at the time of upper layer neuron migration termination, followed by their isolation and proteomic analysis. Generating microglia-like cells from patient-derived stem cells has permitted the opportunity to study specific effects of genotype on phenotype and identified disparate phenotypes of human iPSC-derived microglia driven by genotype and functional effects of microglia in co-cultures and cortical organoids. S23-02 Striatal astrocyte-neuron interactions in health and disease Baljit S Khakh I Department of Physiology UCLA, Los Angeles, USA i Astrocytes tile the central nervous system, but their functions in neural circuits and their roles in mammalian behaviour and disease are incompletely defined. [Extracted from the article] |
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