Initial characterization of potential proteolytic enzyme in crude extract of terong para Solanum virginianum.

Autor: Saputri, Dinar Suksmayu, Mikhratunnisa, Yanti, Sahri, Rosmayana, Anita, Insani, Sri Rahmatul, Lestari, Mimin, Walfadiah, Nurul
Předmět:
Zdroj: AIP Conference Proceedings; 2022, Vol. 2638 Issue 1, p1-8, 8p
Abstrakt: Terong para fruit (para eggplant), a wild plant that is rarely explored scientifically, was extracted by a simple extraction method to analyze its proteolytic activity at several incubation temperature variations, pH, drying temperature, ripeness, and fruit parts. Sumbawa people use para eggplant fruit to make a traditional dessert named palopo. We also analyzed the milk-clotting activity of para eggplant crude extract to explore its potential as a rennet substitute. The recent study found that the highest activity in fresh fruit crude extracts was in ripe fruit seeds incubated at 60 °C pH 7 (2.607 U/mL). After going through the drying process at 50 °C, para eggplant crude extract's proteolytic activity increased to 22.547 U/mL. The milk-clotting activity of para eggplant crude extract was 1.322 U/mL, but it could not coagulate horse milk in the given time of analysis. Further purification and characterization are necessary for this potential protease. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index