| Abstrakt: |
Vanillin is a valuable commodity in various industries. Therefore, its production is carried out on a large scale. The vanillin production from vanilla pods is too expensive and insufficient. In order to meet market needs, vanillin is also produced synthetically. However, due to the high consumer demand for natural or organic food production, many efforts were made to produce natural vanillin through the biotransformation process. Five Bacillus sp. bacteria were obtained from the Spent Bleaching Earth waste. They showed the ability to produce vanillin, whose biotransformation capacity was identified and analyzed by High-Performance Liquid Chromatography. Bacterial identification was performed by using 16S rRNA gene amplification, sequencing, and data analysis using the Geneious program. The DNA sequences were aligned with a database on the NCBI website using the BLAST program. The nucleotide sequences of bacterial were processed using ClustalW and Bioedit. Then, phylogenetic trees were prepared using MEGA-X for relationship analysis. The results of the identification of the five isolates were Bacillus cereus strain SBMAX30 (isolate B1), Bacillus thuringiensis strain LDC 507 (isolate B2), Bacillus thuringiensis strain LB61 (isolate B3), Bacillus cereus strain 190103- R03 (isolate B4) and Bacillus cereus strain LB87 (isolate B5). Vanillin production for 72 hours from 1% (v/v) isoeugenol for isolate B1 was 0.421 ± 0.0337 g/L vanillin (4.28%), isolate B2 was 0.420 ± 0.0679 g/L vanillin (4.28 %), isolate B3 as much as 0.315 ± 0.0983 g/L vanillin (3.20%), isolate B4 as much as 0.168 ± 0.0225 g/L vanillin (1.71%), and isolate B5 as much as 0.386 ± 0.1019 g/L vanillin (3.93%). The highest vanillin production was obtained from isolate B1(Bacillus cereus strain SBMAX30). The result highlights the potential use of bacteria as a platform to produce vanillin, although its practical use needs to be optimized. [ABSTRACT FROM AUTHOR] |
