| Autor: |
Mølvig, J., Baek, L., Cchristensen, P., Manogue, K. R., Vlassara, H., Platz, P., Nielsen, L. S., Svejgaard, A., Nerup, J. |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology; Jun1988, Vol. 27 Issue 6, p705-716, 12p |
| Abstrakt: |
The secretions of interleukin 1 (IL-1), tumour necrosis factor a (TNF), and prostaglandin E2 (PGE;) of low-dose E. coli lipopolysaccharide (LPS)-stimulated human monocytes (Mφ) were investigated in an endotoxin (ET)-free milieu (<1.6 pg LPS/ml). Human Mφ cultures from nine healthy men were stimulated with 0, 12..5-500, and 250,000 pg LPS/ml as measured by a very sensitive Limulus test. The IL-1 activity was tested by the mouse costimulatory thymocyte (LAF) assay, which was thoroughly standardized and characterized (interassay variation 12-24%, intra-assay variation 3-7%). Spontaneous Mφ secretions of IL-1, TNF, and PGE2 were negligible, but 12.5 pg LPS/ml significantly stimulated the secretions of these Mφ products and the monokine responses to 500 and 250, 000 pg LPS/ml were almost in the same range. Il was demonstrated that the secretions of IL-l-TNF and TNF-PGE2 were strongly correlated. Pronounced interindividual differences in LPS responsiveness were demonstrated, and two low-responders, one of whom was HLA-DR 1,2-positive, were identified. Three first-degree relatives of the DRL,2-positive low-responder had similar low responses. Furthermore, Mφ cultures were prepared weekly for 4 weeks from four HLA-DR different men and the only DR2,2 homozygous individual had low monokine responses. In conclusion, stable interindividual differences in in vitro monokine and PGE; secretions of LPS-stimulated Mφ were demonstrated. It is suggested that HLA-DR2-positive individuals may be low responders. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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