| Abstrakt: |
Melanins are diverse pigments ubiquitous in unicellular bacteria to highly advanced eukaryotes. These hydrophobic negatively charged molecules differ extensively in their functionality among species for their free radical scavenging activity as well as oxidative stress reduction highlighting the antiaging property. In this study, nanomelanin synthesized by marine Pseudomonas stutzeri BTCZ109, was critically evaluated for bioproduction and physicochemical characterization using SEM, UV spectroscopy, FT-IR analysis, 1H NMR, and CHNS analysis. Furthermore, the biosynthetic pathway was elucidated using inhibitors including kojic acid, sulcotrione, and sodium azide. The nanomelanin was further evaluated to determine its size, colloidal, and thermal stability by using TEM, particle size analysis, zeta potential, and thermogravimetric analysis, respectively. The biological function of this enigmatic nanomolecule was assessed in terms of antioxidant property using DPPH assay and antibiofilm activity against different biofilm-forming bacteria including Pseudomonas aeruginosa and Bacillus pumilus. [ABSTRACT FROM AUTHOR] |
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