| Autor: |
van de Wiel, A., Seifert, W. F., van der Linden, J. A., Gmelig-Meyling, F. H. J., Kater, L., Schuurman, H.-J. |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology; Feb1987, Vol. 25 Issue 2, p181-187, 7p |
| Abstrakt: |
Changes in the concentration and composition of serum immunoglobulin A (IgA) and deposits of IgA in tissues are well-known characteristics of alcoholic liver disease. We investigated whether these changes also accompany IgA synthesis by peripheral blood mononuclear cells (MNC), by counting immunoglobulin-producing cells using a solid-phase enzymatic ‘spot’ test, and by analysis of immunoglobulin content in lysed cells with culture supernatant using conventional enzymatic methods. Patients with alcoholic liver disease exhibited a significantly higher number of spontaneously IgA-producing cells than did normal healthy controls (1.7×106 cells/l blood and 0.5×106 cells/l blood, respectively, P<0.01). The IgA content of MNC directly after isolation was also higher (38 and 13 ng/106 MNC, respectively, P<0.01), as was the IgA production during an unstimulated 6-day culture period (520 and 95 ng/106 MNC put into culture, respectively, P<0.001). The spontaneously IgA-producing cells assessed directly after isolation of mononuclear cells correlated with the IgA production during an unstimulated culture (P<0.01). We conclude that in alcoholic liver disease, B lymphocytes circulate which may have been activated in vivo. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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