| Autor: |
MacKay, Gillian E., Keighren, Margaret A., Wilson, Linda, Pratt, Thomas, Flockhart, Jean H., Mason, John O., Price, David J., West, John D. |
| Předmět: |
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| Zdroj: |
Journal of Anatomy; Jan2005, Vol. 206 Issue 1, p79-92, 14p |
| Abstrakt: |
The mouseTgTP6.3transgene, encoding a tauGFP fusion protein, is becoming widely used but has yet to be fully characterized and evaluated as suitable lineage marker. The aim of the present study was to investigate the phenotype ofTgTP6.3 +/+ homozygotes andTgTP6.3 +/– hemizygotes, characterize the expression of theTgTP6.3transgene in different tissues and critically evaluate its use as a lineage marker.TgTP6.3 +/+ homozygotes died between embryonic day 14.5 and weaning, whereasTgTP6.3 +/– hemizygotes were mostly viable and fertile but smaller than non-transgenic siblings.TgTP6.3expression began in the late two-cell stage, persisted in most fetal and adult tissues and was uniformly expressed in many (but not all) tissues.TgTP6.3 +/– cells were readily identified in many chimeric tissues and their contribution appeared to be quantitatively and spatially normal. Overall, tauGFP expression in hemizygousTgTP6.3 +/– cells fulfils the main criteria of a good lineage marker for many tissues. It provides a useful lineage marker, which should be particularly suitable for axons, blood vessels and pre-implantation embryos. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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