| Abstrakt: |
Phytochemical investigation of corn silk resulted in isolation and characterization of four flavone C-glycosides, chrysoeriol 6- C - β -oliopyranosyl-7- O - β -D-glucopyranoside (1), 3′-methoxycassiaoccidentalin A (2), chrysoeriol 6- C - β -boivinopyranosyl-7- O - β -D-glucopyranoside (3), and ax-4″-OH-3′-methoxymaysin (4), a triterpenoid, friedelin (5), two sterols, (22 E)-5 α ,8 α -epidioxyergosta-6,22-dien-3 β -ol (6) and 6 β -hydroxystigmasta-4,22-diene-3-one (7), and a mixture of β -sitosterol and stigmasterol. Compounds 1 and 2 were previously undescribed. Structure elucidation of the isolated compounds was attained using spectral data including 1D and 2D NMR and HRESIMS. Compounds 1, 2, 5 , and 6 inhibited iNOS activity in LPS-induced macrophages and decreased nitrite levels by 68.64 ± 4.46, 65.67 ± 6.47, 88.50 ± 0.50, and 94.00 ± 4.00%, respectively, at 50 µM. Compound 5 also showed inhibition of NF- κ B (51.00 ± 1.50%). Compounds 1 and 2 induced NAG-1 activity in chondrocytes by 1.80 ± 0.05 and 2.00 ± 0.13 fold, respectively. The extract of corn silk, however, did not exhibit inhibition of iNOS or NF- κ B but induced NAG-1 by 1.80 ± 0.51 fold. [ABSTRACT FROM AUTHOR] |
