Genome-wide functional analysis reveals key roles for kinesins in the mammalian and mosquito stages of the malaria parasite life cycle.

Autor: Zeeshan, Mohammad, Rashpa, Ravish, Ferguson, David J. P., Abel, Steven, Chahine, Zeinab, Brady, Declan, Vaughan, Sue, Moores, Carolyn A., Le Roch, Karine G., Brochet, Mathieu, Holder, Anthony A., Tewari, Rita
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Zdroj: PLoS Biology; 7/28/2022, Vol. 20 Issue 7, p1-34, 34p, 7 Color Photographs
Abstrakt: Kinesins are microtubule (MT)-based motors important in cell division, motility, polarity, and intracellular transport in many eukaryotes. However, they are poorly studied in the divergent eukaryotic pathogens Plasmodium spp., the causative agents of malaria, which manifest atypical aspects of cell division and plasticity of morphology throughout the life cycle in both mammalian and mosquito hosts. Here, we describe a genome-wide screen of Plasmodium kinesins, revealing diverse subcellular locations and functions in spindle assembly, axoneme formation, and cell morphology. Surprisingly, only kinesin-13 is essential for growth in the mammalian host while the other 8 kinesins are required during the proliferative and invasive stages of parasite transmission through the mosquito vector. In-depth analyses of kinesin-13 and kinesin-20 revealed functions in MT dynamics during apical cell polarity formation, spindle assembly, and axoneme biogenesis. These findings help us to understand the importance of MT motors and may be exploited to discover new therapeutic interventions against malaria. A comprehensive study reveals that kinesins in the malaria parasite Plasmodium have diverse cellular roles and locations, including functions in spindle assembly during proliferation, axoneme formation in flagellum biogenesis, and determining the apical morphology of the cell. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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