| Autor: |
Liu, Zhenyang, Wen, Sitao, Wu, Guogan, Wu, Huawei |
| Předmět: |
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| Zdroj: |
European Food Research & Technology; Sep2022, Vol. 248 Issue 9, p2311-2324, 14p |
| Abstrakt: |
The rumen of ruminants contains a variety of fungi with function of producing xylanases to break down plant cell walls. In this study, a new glycoside hydrolase family 10 (GH10) xylanase gene ArXyn10c20 from anaerobic rumen microorganism Anaeromyces robustus was successfully synthesized and expressed in Pichia pastoris GS115, with a protein molecular weight of approximately 42 kDa. The optimum pH and temperature for ArXyn10c20 were 5.5 and 40 ℃. ArXyn10c20 was stable in the pH range 5.0–9.0, retaining over 75.0% relative activity for 1 h. The activity of recombinant xylanase was significantly enhanced by 1 mM Cu2+. The products of ArXyn10c20 hydrolysis of beechwood xylan were xylobiose, xylotriose and xylotetraose by thin-layer chromatography analysis. In food applications, ArXyn10c20 can significantly improve the quality of dough and bread. With the addition of 420 U of ArXyn10c20 per 100 g flour, the hardness, gumminess and chewiness of the bread decreased by 42.2%, 45.3%, and 55.4%, respectively, and the reducing sugar increased by 18.7%. The new discovered xylanase ArXyn10c20 has great potential in food industry. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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