| Autor: |
Qiu, Kangqiang, Zou, Weiwei, Fang, Hongbao, Hao, Mingang, Mehta, Kritika, Tian, Zhiqi, Guan, Jun-Lin, Zhang, Kai, Huang, Taosheng, Diao, Jiajie |
| Předmět: |
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| Zdroj: |
Nature Communications; 7/25/2022, Vol. 13 Issue 1, p1-9, 9p |
| Abstrakt: |
Mitochondria are highly dynamic organelles whose fragmentation by fission is critical to their functional integrity and cellular homeostasis. Here, we develop a method via optogenetic control of mitochondria–lysosome contacts (MLCs) to induce mitochondrial fission with spatiotemporal accuracy. MLCs can be achieved by blue-light-induced association of mitochondria and lysosomes through various photoactivatable dimerizers. Real-time optogenetic induction of mitochondrial fission is tracked in living cells to measure the fission rate. The optogenetic method partially restores the mitochondrial functions of SLC25A46−/− cells, which display defects in mitochondrial fission and hyperfused mitochondria. The optogenetic MLCs system thus provides a platform for studying mitochondrial fission and treating mitochondrial diseases. Existing methods can lack spatiotemporal accuracy to manipulate dynamic mitochondrial behaviour in live cells. Here the authors report an optogenetic method to control mitochondria-lysosome contacts and induce mitochondrial fission; they use photoactivatable dimerizers including CRY2/CIB and SspB/iLID. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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