| Autor: |
Weber, David S., Rocic, Petra, Mellis, Adamantios M., Laude, Karine, Lyle, Alicia N., Harrison, David G., Griendling, Kathy K. |
| Předmět: |
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| Zdroj: |
American Journal of Physiology: Heart & Circulatory Physiology; Jan2005, Vol. 288 Issue 1, pH37-H42, 6p |
| Abstrakt: |
Increased reactive oxygen species (ROS) are implicated in several vascular pathologies associated with vascular smooth muscle hypertrophy. In the current studies, we utilized transgenic (Tg) mice (Tgp22smc) that overexpress the p22phox subunit of NAD(P)H oxidase selectively in smooth muscle. These mice have a twofold increase in aortic p22phox expression and H2O2 production and thus provide an excellent in vivo model in which to assess the effects of increased ROS generation on vascular smooth muscle cell (VSMC) function. We tested the hypothesis that overexpression of VSMC p22phox potentiates angiotensin II (ANG II)-induced vascular hypertrophy. Male Tgp22smc mice and negative littermate controls were infused with either ANG II or saline for 13 days. Baseline blood pressure was not different between control and Tgp22smc mice. ANG II significantly increased blood pressure in both groups, with this increase being slightly exacerbated in the Tgp22smc mice. Baseline aortic wall thickness and cross sectional wall area were not different between control and Tgp22smc mice. Importantly, the ANG II-induced increase in both parameters was significantly greater in the Tgp22smc mice compared with control mice. To confirm that this potentiation of vascular hypertrophy was due to increased ROS levels, additional groups of mice were coinfused with ebselen. This treatment prevented the exacerbation of hypertrophy in Tgp22smc mice receiving ANG II. These data suggest that although increased availability of NAD(P)H oxidase derived ROS is not a sufficient stimulus for hypertrophy, it does potentiate ANG II-induced vascular hypertrophy, making ROS an excellent target for intervention aimed at reducing medial thickening in vivo. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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