EPA and DHA confer protection against deoxynivalenol-induced endoplasmic reticulum stress and iron imbalance in IPEC-1 cells.
| Autor: | Lin, Jia, Huang, Feifei, Liang, Tianzeng, Qin, Qin, Xu, Qiao, Huang, Xingfa, Zhang, Jing, Xiao, Kan, Zhu, Huiling, Zhao, Jiangchao, Liu, Yulan |
|---|---|
| Předmět: |
IRON metabolism
DOCOSAHEXAENOIC acid HOMEOSTASIS SEQUENCE analysis TRANSFERRIN ENDOPLASMIC reticulum RNA FLUORESCENCE spectroscopy MOLECULAR biology MALONDIALDEHYDE ENZYME-linked immunosorbent assay GENETIC techniques POLYMERASE chain reaction REACTIVE oxygen species SPECTROPHOTOMETRY CARRIER proteins |
| Zdroj: | British Journal of Nutrition; 7/28/2022, Vol. 128 Issue 2, p161-171, 11p |
| Abstrakt: | This study assessed the molecular mechanism of EPA or DHA protection against intestinal porcine epithelial cell line 1 (IPEC-1) cell damage induced by deoxynivalenol (DON). The cells were divided into six groups, including the CON group, the EPA group, the DHA group, the DON group, the EPA + DON group and the DHA + DON group. RNA sequencing was used to investigate the potential mechanism, and qRT-PCR was employed to verify the expression of selected genes. Changes in ultrastructure were used to estimate pathological changes and endoplasmic reticulum (ER) injury in IPEC-1 cells. Transferrin receptor 1 (TFR1) was tested by ELISA. Fe2+ and malondialdehyde (MDA) contents were estimated by spectrophotometry, and reactive oxygen species (ROS) was assayed by fluorospectrophotometry. RNA sequencing analysis showed that EPA and DHA had a significant effect on the expression of genes involved in ER stress and iron balance during DON-induced cell injury. The results showed that DON increased ER damage, the content of MDA and ROS, the ratio of X-box binding protein 1s (XBP-1s)/X-box binding protein 1u (XBP-1u), the concentration of Fe2+ and the activity of TFR1. However, the results also showed that EPA and DHA decreased the ratio of XBP-1s/XBP-1u to relieve DON-induced ER damage of IPEC-1 cells. Moreover, EPA and DHA (especially DHA) reversed the factors related to iron balance. It can be concluded that EPA and DHA reversed IPEC-1 cell damage induced by DON. DHA has the potential to protect IPEC-1 cells from DON-induced iron imbalance by inhibiting ER stress. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
| Externí odkaz: |
|