Downregulation of GJB2 and SLC26A4 genes induced by noise exposure is associated with cochlear damage.

Autor: Garmaroudi, Amir Abbasi, Khadem, Monireh, Hotkani, Maryam Mirzaei, Mozaffari, Sajjad, Monazzam Esmaeil poor, Mohammad Reza, Kazemirad, Elham
Zdroj: Molecular Biology Reports; Aug2022, Vol. 49 Issue 8, p7219-7229, 11p
Abstrakt: Background: Noise-induced hearing loss (NIHL) is one the major causes of acquired hearing loss in developed countries. Noise can change the pattern of gene expression, inducing sensorineural hearing impairment. There is no investigation on the effects of noise frequency on the expression of GJB2 and SLC26A4 genes involved in congenital hearing impairment in cochlear tissue. Here we investigated the impacts of white and purple noise on gene expression and pathologic changes of cochlear tissue. Methods: In this study, 32 adult male Westar rats were randomly divided into experimental groups: WN, animals exposed to white noise with a frequency range of 100-20000 Hz; PN, animals exposed to purple noise with a frequency range of 4–20 kHz, and control group, without noise. The experimental groups were exposed to a 118–120 dB sound pressure level for 8 h per 3 days and 6 days. 1 h and 1 week after termination of noise exposure, cochlear tissue was prepared for pathology and gene expression analysis. Results: Both white and purple noises caused permanent damage to the cortical, estrosilica systems of hair cells and ganglion of the hearing nerve. GJB2 and SLC26A4 were downregulated in both groups exposed with white and purple noise by increasing the time of noise exposure. However, differences are notably more significant in purple noise, which is more intensified. Also, 1 weak post noise exposure, the downregulation is remarkably higher than 1 h. Conclusions: Our findings suggest that downregulation of GJB2 and SLC26A4 genes are associated with pathological injury in response to noise exposure in cochlear tissue. It would be suggested the demand for assessment of RNA and protein expression of genes involved in noise-induced hearing loss and subsequently the practice of hearing protection programs. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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