| Abstrakt: |
The creation of varieties resistant to different diseases and changing climate conditions, as well as high-quality berries, is the main goal of grape breeders. One of the approaches is the hybridization of cultivated Vitis vinifera L. varieties with wild and/or ancient accessions. A comprehensive study of the grapes' genetic resources is highly necessary for a choosing of candidates for breeding process. The purposes of our study were: the determination of phylogenetic relationships between the 50 varieties and wild representatives of the Vitis vinifera L. species, compliance checking of the model of ecological and geographical groups to the varieties we have selected, and determination of the origin of some Crimean and other autochthonous grape varieties. The study was conducted on the basis of microsatellite DNA analysis for nine SSR markers approved by Vine and Wine (OIV) (VVS2, VVMD5, VVMD7, VVMD27, VVMD28, VVMD25, VVMD32, VrZAG62, VrZAG79) using the technology of genetic identification of grape varieties developed and published earlier. This technology includes multiplex PCR for all markers followed by the electrophoretic analysis of the obtained DNA fragments in one capillary of a genetic analyzer. For each studied sample, a genetic profile was obtained in the form of an individual set of lengths of DNA fragments, which allowed us to use the mathematical method of "Neighbor Joining" to construct a dendrogram reflecting phylogenetic relationships within our sample of varieties. As a result of our study, it was found that the model of ecological and geographical groups of ancient autochthonous varieties in the studied sample does not reflect the phylogenetic relationships between the samples. The spread of cultivated grape varieties at the initial stage of the development of viticulture went from east to west. The studied autochthonous Crimean grape varieties were introduced from other regions and do not originate from local wild forms of V. vinifera L. [ABSTRACT FROM AUTHOR] |
Full text from SpringerLink