| Autor: |
Farstad, I. N., Halstensen, T. S., Fausa, O., Brandtzaeg, P. |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology; Nov1993, Vol. 38 Issue 5, p451-458, 8p |
| Abstrakt: |
T-cell receptor γ/&delta+ (TcRγ/&delta+) lymphocytes in human Peyer's patches (PP) adjoining ileal mucosa were studied by monoclonal antibodies with paired immunofluorescence staining in situ and by flow-cytometric phenotyping of isolated cells. The proportion of γ/&delta+ T cells in the follicle-associated epithelium outside the M-cell areas (median 4.1%, range 2.2-30.1%) was similar to that in mucosal villous epithelium (median 4.4%, range 0.5-30.5%). Most intraepithelial γ/&delta) cells (∼90%) expressed CD45R0 but only a few expressed CD8 (< 10%) and none L-selectin; a dominating subset (median 46%) employed the V&delta1/J&delta1 gene product (range 22-100%). The M-cell areas lacked γ/δ cells but contained clustered CD20+ and CD3+ lymphocytes. The subepithelial PP dome area and interfollicular (T-cell) zones, as well as the mucosal lamina propria, contained very few γ/&delta cells (median 1.7%, range 0.4-8.9%.) which were dominated (88-100%) by the Vδ2-encoded subset. Those in the dome area and lamina propria were often (∼75%) CD45R0+ (range 44-90%) while very few (∼2%) expressed L-selectin (range 0-15%). By contrast, CD45R0 expression on γ/&delta cells in the PP T-cell zones was relatively low (∼46%) and that of L-selectin relatively high (∼43%). In conclusion, TcR γ/&delta+ cells are quite rare in human PP and belong mainly to the V&delta2-encoded subset, thus being different from most intraepithelial γ/&delta cells that probably have another origin. The L-selectin+ fraction of PP γ/&delta cells presumably represent newly recruited 'naive' T lymphocytes while CD45R0+ γ/&delta cells both in PP and lamina propria are probably antigen-primed. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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