| Autor: |
Trujillo, Miguel A., Roux, Danièle, Fueri, Josette P., Samuel, Denise, Cailla, Hélène L., Rickenberg, Howard V. |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; 11/16/87, Vol. 169 Issue 1, p167-173, 7p |
| Abstrakt: |
The use of a highly specific radioimmunoassay and of HPLC permitted us to confirm the occurrence of 2'-5' oligoadenylates [px(A2'p5') nA] in several strains of Escherichia coli. Cellular concentrations of 2'-5' oligoadenylates ranged from 50 nM to 300 nM, The mixture of 2'-5' oligoadenylates consisted primarily of pppA2'p5'A, pA2'p5'A, A2'p5'Ap and A2'p5'A under normal conditions of growth. None of them activated RNase L, Infection of the bacteria with the single-stranded DNA phage Ml 3 or induction of a heat-inducible, non-lytic mutant of phage A led to a significant increase in the total pool of 2'-5' oligoadenylates, paralleling the progressive inhibition of growth. Likewise, the inhibition of protein synthesis with chloramphenicol stimulated the accumulation of 2'- 5' oligoadenylates. Furthermore, the inhibition of bacterial growth by either phage or by chloramphenicol brought about a change in the composition of the 2'-5' oligoadenylate pool; 5'-phosphorylated 2'-5' oligoadenylates accumulated and became the major components. The findings indicate a parallelism between the effects of viral infection on the synthesis of 2'-5' ohgoadenylates in eukaryotes and similar effects subsequent to phage growth in the bacterium E. coli. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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