| Autor: |
Köhler, Georges |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology; Feb1993, Vol. 37 Issue 2, p120-129, 10p |
| Abstrakt: |
This article discusses derivation and diversification of monoclonal antibodies. A mouse can make ten million different antibodies, each synthesized by its own B lymphocyte. When a conventional mouse response to such a determinant is analysed, only 5-10 different antibody species are seen, representing probably a random sample of the total repertoire. Mouse myeloma tumour cells are fused to spleen cells derived from a mouse which had previously been immunized with antigen. About 50% of the hybrid cells combine the hoped for parental traits: vigorous growth in tissue culture derived from the myeloma tumour cell and antibody production coming from the splenic B cell. |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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