Comparison of the Mannan Structure from the Cell-Wall Mutant Candida sp. M-7002 and Its Wild Type.

Autor: Hamada, Takashi, Nakajima, Tasuku, Izaki, Kazuo, Matsuda, Kazuo
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Zdroj: European Journal of Biochemistry; 10/1/81, Vol. 119 Issue 2, p365-371, 7p
Abstrakt: The cell-wall mutant of a hydrocarbon-assimilating yeast, Candida sp. M-7002 and its wild type have shown a significant difference in mannose content. Each mannan was isolated from the mutant and the wild-type cells by fractionation with Cetavion and copper reagent. Both mannans contain D-mannose, D-glucose and phosphate. The mutant mannan has a relatively high content of protein (1870 in weight bases) whereas the wild-type mannan has a low protein content (5.1%) with a high amount of carbohydrate (>90%). Structural analyses by enzymatic and chemical methods showed that both mannans had an mannosidic (α1 -6)-linked back bone substituted at O-2 by side chains of varying length. The side chains of the mutant mannan were shown to consist of single niannose units and disaccharide units whose linkages were predominantly α1 -2. while the wild-type mannan had two additional side chains of disaccharides. These additional side chains had α1-3 linkages which were scarcely found in the mutant mannan. β-Elimination reaction demonstrated that the mannans also contain mannosyl oligosaccharides linked to protein through O-glycosidic linkage. The chemical properties of the mannan of the Candida mutant indicates that the mutation might occur not only in the side chain structure but also in the (α1 -6)-linked mannan back bone. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index