| Abstrakt: |
An expression system for the production of recombinant T-cell receptor (TCR) variable domains Would, inter alia, allow structural studies lo be carried out and provide protein for the generation of anti-clonotypic antibodies. In this report the Vα and Vβ domain genes have been isolated from a T-cell hybridoma which is associated with the pathogenesis of experimental allergic encephalomyelitis (EAE) in the H-2umotise. These have been expressed as secreted domains in Eschierichia coli, using secretion vectors previously used for the production of immunoglobulin fragments. Both Vα and Vβ domains are secreted in milligram quantities into the culture supernatant, although the levels of the Vα domain are about 10-20 fold higher than those of the Vα domain. This expression system offers a rapid route for the production of recombinant TCRs in soluble form. [ABSTRACT FROM AUTHOR] |
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