| Autor: |
Alonso, J. M., Rodriguez, J., Viñuela, E., Kroemer, G., Martínez-A., C. |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology; Nov1991, Vol. 34 Issue 5, p619-626, 8p |
| Abstrakt: |
Using a recombinant vaccinia virus (VV) that expresses E. coli β galactosidase (β-Gal) lo infect lymphocytes, we show that enzymometrically or immunologically delectable β-Gal expression is less pronounced among T cells than among B cells. VV infection caused growth inhibition of B cells, but barely affected T-cell proliferation in vitro. Moreover, the production of infectious viral particles was less pronounced in T lymphocytes. Kinetic studies revealed that after an initial dose-dependent growth inhibition, T cells continued lo proliferate without the doubling time being affected by VV infection. Nonetheless, the T cells do express proteins encoded by recombinant VV, such as β-Gal. or secrete soluble proteins such as interleukin-4 though at a lower efficiency at the per cell level than B lymphocytes. In conclusion, the physiology of T cells appears to be less perturbed by VV than that of B cells, although the virus is capable of directing expression of recombinant genes to T lymphocytes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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