| Autor: |
Forest, Chiara, Orduña, Luis, Matus, José Tomás, Tornielli, Giovanni Battista, Zenoni, Sara, Amato, Alessandra |
| Předmět: |
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| Zdroj: |
Julius-Kühn-Archiv; Jul2022, Issue 470, p118-118, 1p |
| Abstrakt: |
NACs (NAM/ATAF/CUC) are plant-specific transcription factors (TFs) and represent an interesting family due to their key role in plant development processes and stress responses. Among the 74 Vitis vinifera family members, VviNAC61 was selected for functional characterization as one of the key candidates of the major transcriptome reprogramming occurring during grapevine development and for its role as a molecular marker of the grape postharvest withering process. VviNAC61 is particularly expressed at veraison and post-veraison berry stages and shows a high expression also in lignified tissues such as roots, tendrils, seeds and rachis. In leaves, VviNAC61 achieves its higher level of expression in the senescence stage; in line with this observation, a NAC family multispecies phylogenetic tree revealed that VviNAC61 clusters with VviNAC33 and AtANAC046, both involved in the senescence processes. The effect of VviNAC61 over-expression was investigated through the agroinfiltration of N. benthamiana leaves, showing clear wilting symptoms and altered stomatal conductance. A transcriptomic analysis was performed on cv. 'Sultana' leaves transiently overexpressing VviNAC61 and a search of TF-bound genes by DNA Affinity Purification and sequencing (DAP-seq) was performed to narrow down the list of VviNAC61-regulated candidate genes. The "secondary metabolism", "transcription facor regulation" and "oxidative stress" functional categories were found as mainly enriched among candidate target genes. More in detail, several laccases and genes encoding for the phenylpropanoid pathway-related enzymes, senescence-related genes and several MYB and NAC genes (including VviNAC61) were bound in their promoter region by this TF. The control of the stilbene synthase regulator VviMYB14, the chlorophyll degradation regulator VviSGR1, as well as the VviNAC61 self regulation were validated through the luciferase assay. As last, the upstream regulation mechanism of VviNAC61 was further investigated demonstrating that VviNAC61 is controlled and interacts with CARPO (VviNAC60), a previously characterized master regulator of grapevine organ maturation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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