| Autor: |
Larrick, J. W., Coloma, M. J., Del Valle, J., Fernandez, M. E., Fry, K. E., Gavilondo-Cowley, J. V. |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology; Aug1990, Vol. 32 Issue 2, p121-128, 8p |
| Abstrakt: |
C6 is a potentially therapeutic murine monoclonal antibody that recognizes the class 1 outer membrane protein of Neisseria meningitidis. C6 specifically immunoblots this antigen and augments in vitro killing of N. meningitidis bacteria. We describe a general method of obtaining the heavy and light chain variable-region sequence from immunoglobuhn-secreting cells. The method uses mixed polymerase chain reaction (PCR) primers designed from the 5'end of the framework I (FRI) sequences of the heavy and light chains, and 3-end primers for constantregion conserved sequences. The method has been applied to the cloning and sequencing of the variable region of C6 to construct a humanized monoclonal antibody. Rapid amplification and sequencing of variable regions by this general method have multiple applications in the study of the immune response to infectious diseases. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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