| Autor: |
Lima, Alex Ranieri Jerônimo, Silva, Herbert Guimarães de Sousa, Poubel, Saloe, Rosón, Juliana Nunes, de Lima, Loyze Paola Oliveira, Costa-Silva, Héllida Marina, Gonçalves, Camila Silva, Galante, Pedro A. F., Holetz, Fabiola, Motta, Maria Cristina Machado, Silber, Ariel M., Elias, M. Carolina, da Cunha, Julia Pinheiro Chagas |
| Předmět: |
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| Zdroj: |
Epigenetics & Chromatin; 7/6/2022, Vol. 15 Issue 1, p1-16, 16p |
| Abstrakt: |
Background: Genomic organization and gene expression regulation in trypanosomes are remarkable because protein-coding genes are organized into codirectional gene clusters with unrelated functions. Moreover, there is no dedicated promoter for each gene, resulting in polycistronic gene transcription, with posttranscriptional control playing a major role. Nonetheless, these parasites harbor epigenetic modifications at critical regulatory genome features that dynamically change among parasite stages, which are not fully understood. Results: Here, we investigated the impact of chromatin changes in a scenario commanded by posttranscriptional control exploring the parasite Trypanosoma cruzi and its differentiation program using FAIRE-seq approach supported by transmission electron microscopy. We identified differences in T. cruzi genome compartments, putative transcriptional start regions, and virulence factors. In addition, we also detected a developmental chromatin regulation at tRNA loci (tDNA), which could be linked to the intense chromatin remodeling and/or the translation regulatory mechanism required for parasite differentiation. We further integrated the open chromatin profile with public transcriptomic and MNase-seq datasets. Strikingly, a positive correlation was observed between active chromatin and steady-state transcription levels. Conclusion: Taken together, our results indicate that chromatin changes reflect the unusual gene expression regulation of trypanosomes and the differences among parasite developmental stages, even in the context of a lack of canonical transcriptional control of protein-coding genes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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