Measuring Vitamin D 3 Metabolic Status, Comparison between Vitamin D Deficient and Sufficient Individuals.

Autor: Castillo-Peinado, Laura de los Santos, Calderón-Santiago, Mónica, Herrera-Martínez, Aura Dulcinea, León-Idougourram, Soraya, Gálvez-Moreno, María Ángeles, Sánchez-Cano, Rafael Luis, Bouillon, Roger, Quesada-Gómez, Jose Manuel, Priego-Capote, Feliciano
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Zdroj: Separations (2297-8739); Jun2022, Vol. 9 Issue 6, pN.PAG-N.PAG, 15p
Abstrakt: The main branch of vitamin D3 metabolism involves several hydroxylation reactions to obtain mono-, di- and trihydroxylated metabolites, including the circulating and active forms—25(OH)D3 and 1,25(OH)2D3, respectively. However, most clinical trials strictly target the determination of 25(OH)D3 to offer a view of the metabolic status of vitamin D3. Due to the growing interest in expanding this restricted view, we have developed a method for measuring vitamin D3 metabolism by determination of vitamin D3, 25(OH)D3, 24,25(OH)2D3, 1,25(OH)2D3 and 1,24,25(OH)3D3 in human plasma. The method was based on SPE–LC–MS/MS with a large volume injection of human plasma (240 µL). Detection of di- and trihydroxymetabolites, found at the picogram per milliliter level, was attained by the combined action of high preconcentration and clean-up effects. The method allows obtaining information about ratios such as the known vitamin D metabolite ratio (24,25(OH)2D3/25(OH)D3), which can provide complementary views of vitamin D3 metabolic status. The method was applied to a cohort of obese patients and a reference cohort of healthy volunteers to find metabolic correlations between target analytes as well as differences as a function of vitamin D levels within and between cohorts. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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