| Autor: |
Mauney, J. R., Hillman, W. S., Miller, C. O., Skoog, F., Clayton, R. A., Strong, F. M. |
| Předmět: |
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| Zdroj: |
Physiologia Plantarum; 1952, Vol. 5 Issue 4, p485-497, 13p |
| Abstrakt: |
A bioassay and a procedure for the purification of a growth factor from coconuts have been worked out. The purification involved extraction of the meat with water, removal of proteins by heat and alcohol denaturation, and separation of the fat. Precipitation of the factor by the method of Neuberg, followed by electrodialysis yielded an active basic fraction which was shown by paper chromatography to contain at least four ninhydrin-positive compounds, one of which gave a positive Sakaguchi test for guanido compounds. Evidence to date indicates that the growth factor from coconuts is a heat stabile, acid- and alkali-labile, non-volatile, water-soluble organic compound. Since the factor moves to the cathode from an aqueous solution during electrodialysis, it seems to have basic properties. A concentrate of the active constituent of coconut meat has been prepared which is 4350 times more active on a fresh weight basis than the starting material. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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