Simple synthesis of massively parallel RNA microarrays via enzymatic conversion from DNA microarrays.

Autor:	Schaudy, Erika, Hölz, Kathrin, Lietard, Jory, Somoza, Mark M.
Předmět:	DNA microarrays CATALYTIC RNA RNA NUCLEOTIDE sequence SMALL molecules DNA primers
Zdroj:	Nature Communications; 6/30/2022, Vol. 13 Issue 1, p1-9, 9p
Abstrakt:	RNA catalytic and binding interactions with proteins and small molecules are fundamental elements of cellular life processes as well as the basis for RNA therapeutics and molecular engineering. In the absence of quantitative predictive capacity for such bioaffinity interactions, high throughput experimental approaches are needed to sufficiently sample RNA sequence space. Here we report on a simple and highly accessible approach to convert commercially available customized DNA microarrays of any complexity and density to RNA microarrays via a T7 RNA polymerase-mediated extension of photocrosslinked methyl RNA primers and subsequent degradation of the DNA templates. RNA microarrays have many potential applications, but are difficult to produce. Here, the AUs present a method for converting commercial, customizable DNA microarrays into RNA microarrays using an accessible three-step process involving primer photocrosslinking, extension, and template degradation. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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