Pancreatic α and β cells are globally phase-locked.

Autor: Ren, Huixia, Li, Yanjun, Han, Chengsheng, Yu, Yi, Shi, Bowen, Peng, Xiaohong, Zhang, Tianming, Wu, Shufang, Yang, Xiaojing, Kim, Sneppen, Chen, Liangyi, Tang, Chao
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Zdroj: Nature Communications; 6/29/2022, Vol. 13 Issue 1, p1-16, 16p
Abstrakt: The Ca2+ modulated pulsatile glucagon and insulin secretions by pancreatic α and β cells play a crucial role in glucose homeostasis. However, how α and β cells coordinate to produce various Ca2+ oscillation patterns is still elusive. Using a microfluidic device and transgenic mice, we recorded Ca2+ signals from islet α and β cells, and observed heterogeneous Ca2+ oscillation patterns intrinsic to each islet. After a brief period of glucose stimulation, α and β cells' oscillations were globally phase-locked. While the activation of α cells displayed a fixed time delay of ~20 s to that of β cells, β cells activated with a tunable period. Moreover, islet α cell number correlated with oscillation frequency. We built a mathematical model of islet Ca2+ oscillation incorporating paracrine interactions, which quantitatively agreed with the experimental data. Our study highlights the importance of cell-cell interaction in generating stable but tunable islet oscillation patterns. The Ca2+ modulated pulsatile glucagon and insulin secretions by pancreatic α and β cells are critical in glucose homeostasis. Here the authors show that the Ca2+ oscillations of α and β cells are phase-locked, and that the oscillation pattern is tuned by paracrine interactions between α and β cells. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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