| Abstrakt: |
Finding novel and efficient compounds to be used against cancers is a global research concern. Conjugation of magnetic compounds to silver nanoparticles (NPs) could provide novel characteristics for the nanocomposite. In this work, TiFe2O4@Ag NPs were synthesized, and their physicochemical properties were investigated using FT-IR, XRD, EDX, Zeta potential, VSM, DLS, and electron microscopy analyses. Finally, anticancer potential of the NPs was evaluated using the cellular and molecular assays. The results showed the proper synthesis of the spherical NPs with the size range of 20–60 nm. The nanocomposite had good stability, with the zeta potential of -47.7 mV, and lack of elemental impurities. The MTT assay revealed that the TiFe2O4@Ag NPs were significantly more toxic toward the AGS gastric cancer cells (IC50 = 69.6 µg/mL), than normal HEK293 cells (IC50 = 130 µg/mL). Flow cytometry analysis showed apoptosis induction among NPs treated cells than controls with the frequency of 12.1 and 9.82% for the early and late apoptosis, respectively. In agreement with the flow cytometry and MTT assays, we found that the activity of caspase-3 increased to 1.81 folds, compared with the control cells. Also, the expression of the Bax and p53 genes was increased by 1.4 and 1.6 folds, while the Bcl-2 gene was reduced to 0.7 folds, in comparison with the controls. Our study presented TiFe2O4@Ag NPs as novel and promising anticancer agent which need further characterization to be used in biomedical applications. [ABSTRACT FROM AUTHOR] |
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