| Autor: |
Niyompanich, Jitti, Chuysinuan, Piyachat, Pavasant, Prasit, Supaphol, Pitt |
| Předmět: |
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| Zdroj: |
Polymer Bulletin; Jul2022, Vol. 79 Issue 7, p4667-4684, 18p |
| Abstrakt: |
Poly(ε-caprolactone) (PCL) scaffolds with interconnecting pore were fabricated by solvent casting and particulate leaching. Surface modification of a PCL scaffold to improve hydrophilicity was performed via layer-by-layer (LbL) deposition of poly(diallyldimethylammonium chloride) (PDADMAC) and poly(sodium 4-styrenesulfonate) (PSS) and evaluated using aniline blue staining and X-ray photoelectron spectroscopy (XPS). Aniline blue staining was performed to determine the extent of PDADMAC/PSS deposition on the PCL dual-leached scaffold through the inner pores. Peaks attributed to the N 1s orbitals of PDADMAC and the S 2p orbitals of PSS appeared in the XPS spectra of the PCL scaffold. By using LbL deposition, osteopontin (OPN) was immobilized on the top surface. In vitro biological evaluation of the cytotoxicity, cell proliferation, alkaline phosphatase (ALP) activity, and mineralization were performed. The results showed that the OPN-immobilized PCL scaffold was nontoxic and did not promote cell proliferation, but it highly enhanced the osteogenic differentiation of MC3T3-E1 cells including ALP activity and mineral deposition compared to the control. This study revealed that LbL deposition on a PCL scaffold surface is sufficient to enable the local delivery of OPN to improve cell interactions with scaffolds. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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