| Autor: |
Huh, Wook-Kang, Oono, Takashi, Shirafuji, Yoshinori, Akiyama, Hisanori, Arata, Jirô, Sakaguchi, Masakiyo, Huh, Nam-ho, Iwatsuki, Keiji |
| Předmět: |
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| Zdroj: |
Journal of Molecular Medicine; Oct2002, Vol. 80 Issue 10, p678-684, 7p |
| Abstrakt: |
Defensins are cationic antimicrobial peptides with a broad spectrum. Recently human β-defensin 2 (hBD-2) has been isolated from psoriatic skin; however, its exact localization and fate have not been fully understood. We studied the distribution pattern of hBD-2 in skin tissues of psoriasis and other inflammatory skin diseases. In the upper spinous and granular layer of psoriasis vulgaris hBD-2 was present in the cytoplasm. In the horny layer the positive signals were in a basket-weave pattern, indicating possible accumulation of hBD-2 in the intercellular space. The similar pattern of hBD-2 distribution was observed in the lesions of nummular eczema and atopic dermatitis. hBD-2 was not detected in the section of normal elbow and knee skin. When isolated psoriatic scales were stained, hBD-2 was detected in a wrapping paper-like distribution pattern surrounding the corneocytes. In horny layer of psoriatic skin hBD-2 was closely associated or colocalized with elafin, which is known to be in extracellular space, as demonstrated by double staining. Western blot analysis using cultured human keratinocytes detected hBD-2 with an expected size in the conditioned medium and in the cell lysates when stimulated with 5% FCS or IL-α. These results indicate that hBD-2 was synthesized and remained in cytoplasm in the upper spinous and granular layer, and then secreted into intercellular space in the horny layer. This dynamic change in hBD-2 distribution in epidermis is certainly relevant to function as an innate host defense mechanism against invading micro-organisms. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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