| Abstrakt: |
Background: Owing to their strong antimicrobial properties, Helichrysum arenarium (HA), Anzer thyme (AT), and Stevia rebaudiana (SR) have been commonly used in medicine. Aim: This study aimed to evaluate antimicrobial activities of HA, AT, and SR against S. mutans and S. wiggsiae in biofilms formed on primary teeth. Design: Fifty enamel samples were divided into two groups: mono‐species biofilm and two‐species biofilm. Each biofilm group was divided into five subgroups (n = 5): group 1, HA; group 2, AT; group 3, SR; group 4, CHX (positive control); and group 5, distilled water (negative control). Minimum inhibitory concentration and minimum bactericidal concentration were determined. The number of viable microorganisms was counted. The presence of microorganisms was examined using a scanning electron microscope, and mineral analysis was performed using energy‐dispersive X‐ray analysis. Results: In the mono‐species biofilm, CHX was significantly more effective against S. mutans than other groups (p <.001). Furthermore, HA, AT, and SR groups showed significantly lower colony counts of S. mutans than distilled water (p <.05). In the two‐species biofilm group, AT, SR, and CHX were significantly more effective against S. wiggsiae than distilled water (p <.05). Conclusions: HA, AT, and SR have been suggested as effective natural alternatives to CHX against cariogenic bacteria. [ABSTRACT FROM AUTHOR] |
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