| Autor: |
Prates, John Willians Oliveira, Xisto, Mariana Fonseca, Rodrigues, João Vitor da Silva, Colombari, João Pedro Cruz, Meira, Júlia Maria Alves, Dias, Roberto Sousa, Silva, Cynthia Canedo da, de Paula, e Sérgio Oliveira |
| Předmět: |
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| Zdroj: |
Diagnostics (2075-4418); May2022, Vol. 12 Issue 5, pN.PAG-N.PAG, 12p |
| Abstrakt: |
Zika virus (ZIKV) represents a global human health threat and it is related to severe diseases such as congenital Zika syndrome (CZS) and Guillain-Barré syndrome (GBS). There is no vaccine available nor specific antiviral treatment, so developing sensitive, specific, and low-cost diagnostic tests is necessary. Thus, the objective of this work was to produce the Zika virus envelope protein domain III (ZIKV-EDIII) in Komagataella phaffii KM71H and evaluate its potential for diagnostic applications. After the K. phaffii had been transformed with the pPICZαA-ZIKV-EDIII vector, an SDS-PAGE and Western Blot were performed to characterize the recombinant protein and an ELISA to evaluate the antigenic potential. The results show that ZIKV-EDIII was produced in the expected size, with a good purity grade and yield of 2.58 mg/L. The receiver operating characteristic (ROC) curve showed 90% sensitivity and 87.5% specificity for IgM, and 93.33% sensitivity and 82.76% specificity for IgG. The ZIKV-EDIII protein was efficiently produced in K. phaffi, and it has the potential for diagnostic applications. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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