| Autor: |
Conkright, William R., Beckner, Meaghan E., Sahu, Amrita, Qi Mi, Clemens, Zachary J., Lovalekar, Mita, Flanagan, Shawn D., Martin, Brian J., Ferrarelli, Fabio, Ambrosio, Fabrisia, Nindl, Bradley C. |
| Předmět: |
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| Zdroj: |
Journal of Applied Physiology; May2022, Vol. 132 Issue 5, p1125-1136, 12p |
| Abstrakt: |
Extracellular vesicles (EVs) are mediators of physiological changes that occur during physical exertion. This study examined the effects of physical exertion with and without sleep and caloric restriction on EV size, concentration, and surface proteins in men and women. Twenty participants (10 men) completed a 5-day simulated military operational stress protocol with daily physical exertion. Blood was drawn before and immediately after exertion at baseline (D1) and following 48-h of sleep and caloric restriction (D3). EV size and concentration were assessed using nanoparticle tracking analysis. EVs were identified with markers associated with exosomes (CD63), microvesicles (VAMP3), apoptotic bodies (THSD1), and skeletal muscle-derived EVs (SGCA) and quantified using imaging flow cytometry. Interactive and main effects of sex, day, and time on EVs were assessed using three-way ANOVAs. EV concentration declined pre to postexertion in women on D1 and D3 but was stable in men. EV size increased from pre to postexertion and from D1 to D3 in men and women. Physical exertion following sleep and caloric restriction increased CD63þ EV concentration, proportion of total EVs, and CD63 surface protein expression regardless of sex. The proportion of SGCAþ EVs increased in men and women following exertion and from D1 to D3 but was higher in women than in men. No differences were observed in VAMP3þ and THSD1þ EVs. This study identified sexually dimorphic EV profiles in response to various stressors. Further investigations are necessary to determine if dimorphic EV responses affect health and performance outcomes during stress. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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