| Autor: |
Eltobgy, Mostafa M., Zani, Ashley, Kenney, Adam D., Estfanous, Shady, Kim, Eunsoo, Badr, Asmaa, Carafice, Cierra, Daily, Kylene, Whithama, Owen, Pietrzak, Maciej, Webb, Amy, Kawahara, Jeffrey, Eddy, Adrian C., Denz, Parker, Lu, Mijia, K. C., Mahesh, Peeples, Mark E., Jianrong Li, Jian Zhu, Jianwen Que |
| Předmět: |
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| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; 5/24/2022, Vol. 119 Issue 21, p1-12, 19p |
| Abstrakt: |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a worldwide health concern, and new treatment strategies are needed. Targeting inflammatory innate immunity pathways holds therapeutic promise, but effective molecular targets remain elusive. Here, we show that human caspase-4 (CASP4) and its mouse homolog, caspase-11 (CASP11), are up-regulated in SARS-CoV-2 infections and that CASP4 expression correlates with severity of SARS-CoV-2 infection in humans. SARS-CoV-2-infected Casp112/2 mice were protected from severe weight loss and lung pathology, including blood vessel damage, compared to wild-type (WT) mice and mice lacking the caspase downstream effector gasdermin-D (Gsdmd2/2). Notably, viral titers were similar regardless of CASP11 knockout. Global transcriptomics of SARS-CoV-2-infected WT, Casp112/2, and Gsdmd2/2 lungs identified restrained expression of inflammatory molecules and altered neutrophil gene signatures in Casp112/2 mice. We confirmed that protein levels of inflammatory mediators interleukin (IL)-1ß, IL-6, and CXCL1, as well as neutrophil functions, were reduced in Casp112/2 lungs. Additionally, Casp112/2 lungs accumulated less von Willebrand factor, a marker for endothelial damage, but expressed more Kruppel-Like Factor 2, a transcription factor that maintains vascular integrity. Overall, our results demonstrate that CASP4/11 promotes detrimental SARS-CoV-2-induced inflammation and coagulopathy, largely independently of GSDMD, identifying CASP4/11 as a promising drug target for treatment and prevention of severe COVID-19. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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