Epigenetic quantification of immunosenescent CD8+ TEMRA cells in human blood.

Autor: Salumets, Ahto, Tserel, Liina, Rumm, Anna P., Türk, Lehte, Kingo, Külli, Saks, Kai, Oras, Astrid, Uibo, Raivo, Tamm, Riin, Peterson, Hedi, Kisand, Kai, Peterson, Pärt
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Zdroj: Aging Cell; May2022, Vol. 21 Issue 5, p1-14, 14p
Abstrakt: Age‐related changes in human T‐cell populations are important contributors to immunosenescence. In particular, terminally differentiated CD8+ effector memory CD45RA+ TEMRA cells and their subsets have characteristics of cellular senescence, accumulate in older individuals, and are increased in age‐related chronic inflammatory diseases. In a detailed T‐cell profiling among individuals over 65 years of age, we found a high interindividual variation among CD8+ TEMRA populations. CD8+ TEMRA proportions correlated positively with cytomegalovirus (CMV) antibody levels, however, not with the chronological age. In the analysis of over 90 inflammation proteins, we identified plasma TRANCE/RANKL levels to associate with several differentiated T‐cell populations, including CD8+ TEMRA and its CD28− subsets. Given the strong potential of CD8+ TEMRA cells as a biomarker for immunosenescence, we used deep‐amplicon bisulfite sequencing to match their frequencies in flow cytometry with CpG site methylation levels and developed a computational model to predict CD8+ TEMRA cell proportions from whole blood genomic DNA. Our findings confirm the association of CD8+ TEMRA and its subsets with CMV infection and provide a novel tool for their high throughput epigenetic quantification as a biomarker of immunosenescence. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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