| Autor: |
Lorino, G., Angeletti, S., Gherardi, G., De Cesaris, M., Gherardi, L., Battistoni, F., Filadoro, F., Dicuonzo, G. |
| Předmět: |
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| Zdroj: |
European Journal of Clinical Microbiology & Infectious Diseases; May2000, Vol. 19 Issue 5, p388-392, 5p |
| Abstrakt: |
Analysis of bacterial DNA using a polymerase chain reaction performed with broad-range eubacterial 16S rDNA primers may yield a diagnosis of bacterial meningitis in cases where Gram staining of cerebrospinal fluid (CFS), antigen detection techniques or culture fail. Since this PCR technique occasionally gives false-positive results due to contamination of samples or laboratory reagents, a study was performed to establish the diagnostic value of assaying concentrations of tumour necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in 90 CSF samples. A high correlation was found between a positive PCR result and the concentrations of TNF-α and IL-10, indicating that cytokine assays may be used as a confirmatory test. The findings suggested that a combination of the PCR technique, amplicon sequencing and assay of TNF-α and IL-10 concentrations in CSF is a reliable and cost-effective procedure for diagnosis of culture-negative bacterial meningitis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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