The isolation and use of iron-oxidizing, moderately thermophilic acidophiles from the Collie coal mine for the generation of ferric iron leaching solution.

Autor: Kinnunen, P. H.-M., Robertson, W. J., Plumb, J. J., Gibson, J. A. E., Nichols, P. D., Franzmann, P. D., Puhakka, J. A.
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Zdroj: Applied Microbiology & Biotechnology; Feb2003, Vol. 60 Issue 6, p748-753, 6p, 1 Black and White Photograph, 1 Diagram, 2 Charts, 5 Graphs
Abstrakt: Moderately thermophilic, iron-oxidizing acidophiles were enriched from coal collected from an open-cut mine in Collie, Western Australia. Iron-oxidizers were enriched in fluidized-bed reactors (FBR) at 60 °C and 70 °C; and iron-oxidation rates were determined. Ferrous iron oxidation by the microbiota in the original coal material was inhibited above 63 C. In addition to four iron-oxidizers, closely related to Sulfobacillus spp that had been earlier isolated from the 60 °C FBR, one heterotroph closely related to Alicyclobacillus spp was isolated. The Alicyclobacillus sp. isolated from the Collie coal mine tolerated a lower pH than known Alicyclobacillus spp and therefore may represent a new species. The optimum temperature for growth of the iron-oxidizing strains was approximately 50 °C and their maximum temperatures were approximately 60 °C. The FBR was adjusted to operate at 50 °C and was inoculated with all of the isolated iron-oxidizing strains. At 60 °C, an iron-oxidation rate of 0.5 g Fe2+ l–1 h–1 was obtained. At 50 °C, the iron-oxidation rate was only 0.3 g Fe2+ l–1 h–1. These rates compare favourably with the iron-oxidation rate of Acidianus brierleyi in shake-flasks, but are considerably lower than mesophilic iron-oxidation rates. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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