| Autor: |
Doleyres, Y., Paquin, C., LeRoy, M., Lacroix, C. |
| Předmět: |
|
| Zdroj: |
Applied Microbiology & Biotechnology; Oct2002, Vol. 60 Issue 1/2, p168-173, 6p, 2 Charts, 2 Graphs |
| Abstrakt: |
Bifidobacterium longum ATCC 15707 cell production was studied in MRS medium supplemented with whey permeate (MRS-WP) during free-cell batch fermentations and continuous immobilized-cell cultures. Very high populations were measured after 12 h batch cultures in MRS-WP medium controlled at pH 5.5 (1.7±0.5×1010 cfu/ml), approximately 2-fold higher than in non-supplemented MRS. Our study showed that WP is a low-cost source of lactose and other components that can be used to increase bifidobacteria cell production in MRS medium. Continuous fermentation in MRS-WP of B. longum immobilized in gellan gum gel beads produced the highest cell concentrations in the effluent (4.9±0.9×109 cfu/ml) at a dilution rate (D) of 0.5 h–1. However, maximal volumetric productivity (6.9±0.4×109 cfu ml–1h–1) during continuous cultures was obtained at D =2.0 h–1, and was approximately 9.5-fold higher than during free-cell batch cultures at an optimal pH of 5.5 (7.2×108 cfu ml–1h–1). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Full text from SpringerLink