Seropositivity for Coxiella burnetii in suspected patients with dengue in São Paulo state, Brazil.

Autor: França, Danilo Alves de, Mioni, Mateus de Souza Ribeiro, Fornazari, Felipe, de Lima Duré, Ana Íris, Silva, Marcos Vinicius Ferreira, Possebon, Fábio Sossai, Richini-Pereira, Virgínia Bodelão, Langoni, Helio, Megid, Jane
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Zdroj: PLoS Neglected Tropical Diseases; 5/10/2022, Vol. 16 Issue 5, p1-14, 14p
Abstrakt: Q fever and brucellosis are zoonoses that cause fever and other systemic clinical signs in humans; their occurrences are neglected and the differential diagnosis for some diseases is disregarded. This study aimed to investigate the seropositivity for Coxiella burnetii and Brucella spp. antibodies in patients suspected of dengue from 38 municipalities in the state of São Paulo, Brazil. The samples (n = 604) were obtained by convenience from the Adolfo Lutz Institute serum bank. Sera were subjected to an indirect immunofluorescence assay (IFA) using in-house and commercial diagnostic protocols to evaluate C. burnetii positivity. For Brucella spp., sera were subjected to rapid plate serum agglutination with buffered acidified antigen (AAT), slow tube serum agglutination (SAL), and 2-mercaptoethanol (2-ME) techniques. Associations and statistical inferences of the results were performed by logistic regression according to the clinical and demographic variables collected from the patients. Statistical analyses were performed using Statistical Analysis Software (SAS) and associations were considered when p value was <0.05. In all, 129 patients showed positive results for Q fever, indicating a seropositivity of 21.4% (95% CI 18.15–24.85). Patients with 14–20 days of symptoms had 2.12 (95% CI 1.34–3.35) times more chances of being seropositive for Q fever than patients with 7–13 days, and patients with 21–27 days of fever had 2.62 (95% CI 1.27–5.41) times more chances of being seropositive for Q fever than patients with 7–13 days. For the other variables analyzed, there were no significant associations between the groups. No positivity for brucellosis was observed. This is the most comprehensive study of people seropositive for Q fever in São Paulo state and provides additional data for the medical community in Brazil. It is suggested that Q fever may be an important differential diagnosis of febrile illnesses in the region, demanding the government's attention and investment in health. Author summary: Brazil is a tropical country with a warm climate and large animal herds that favor many zoonotic agents that circulate in the territory. Due to the high prevalence of some tropical diseases, such as dengue, malaria, and chikungunya, important zoonoses have been neglected during the clinical evaluation of patients, resulting in misdiagnosis. Examples include Q fever and brucellosis, zoonoses that occur in animals in Brazil and are easily transmitted to humans through contaminated air and food products. There is not much data on these diseases in humans in the country representing their actual health situation. The current knowledge is supported by a few serological surveys developed by research groups and case reports. In this study, sera from patients with fever of unknown origin were positive for Q fever and brucellosis. The authors found that, contrary to brucellosis, Q fever has affected many people in São Paulo, the most populous state in the country. In addition, other findings regarding patient profile and disease behavior have been established. This research highlights the need for surveillance and control of Q fever in humans and animals to prevent acute cases and treat chronic patients. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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