Among Antibody-Like Molecules, Monobodies, Able to Interact with Nucleocapsid Protein of SARS-CoV Virus, There Are Monobodies with High Affinity to Nucleocapsid Protein of SARS-CoV-2 Virus.

Autor:	Khramtsov, Y. V., Ulasov, A. V., Lupanova, T. N., Georgiev, G. P., Sobolev, A. S.
Předmět:	SARS-CoV-2 VIRAL proteins AMINO acid sequence ESCHERICHIA coli MOLECULES
Zdroj:	Doklady Biochemistry & Biophysics; Apr2022, Vol. 503 Issue 1, p90-92, 3p
Abstrakt:	Seven amino acid sequences of antibody mimetics molecules, monobodies, capable of interacting with the nucleocapsid protein of the SARS-CoV virus, were taken from the literature. Nucleotide sequences of monobody genes were obtained by gene synthesis, which were expressed in E. coli and isolated using Ni-NTA chromatography. It was shown by thermophoresis that three of the seven selected antibody-like molecules can interact with high affinity (dissociation constant of tens of nM) with the nucleocapsid protein of the SARS-CoV-2 virus. For the remaining four monobodies, only low affinity binding with a dissociation constant of several μM was found. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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