| Autor: |
Liu, Xiaoyu, Yu, Yunru, Liu, Dechen, Li, Jingbo, Sun, Ji, Wei, Qiong, Zhao, Yuanjin, Pandol, S. J., Li, Ling |
| Zdroj: |
NPG Asia Materials; 1/7/2022, Vol. 14 Issue 1, p1-7, 7p |
| Abstrakt: |
Diabetes mellitus is becoming increasingly prevalent worldwide and needs effective clinical treatment methods. β-Cell replacement therapy has become a safe alternative for diabetes treatment in recent years, and encapsulation methods have been proposed to facilitate this type of therapy. Here, we used coaxial microfluidic electrospray technology to generate microcapsules allowing high cell viability (>90%) with porous alginate shells and β cell-containing cores in less than half an hour. Benefitting from microfluidic electrospray, the sizes of the generated microcapsules were adjustable. The biocompatible porous hydrogel shell not only protected β cells from immune rejection but also allowed the exchange of small molecular nutrients during transplantation, and the liquid core guaranteed the high viability of the encapsulated cells. This constructed living cell biosystem further demonstrated its potential as an artificial islet after transplantation into the omental pouches of diabetic mice to control blood glucose levels and thus treat diabetes. We consider that this system, with an elaborate structure and an abundance of highly viable encapsulated β cells to improve treatment performance, could be applied in a wide range of clinical situations.The β-cell microcapsules prepared by the microfluidic electrospray method were transplanted into the omentum pouch of mice for intelligent release of insulin to treat diabetes. Studies have shown that microcapsules can encapsulate cells while allowing nutrients and metabolites to enter and exit. This ability helps protect cells, improve cell activity, and reduce inflammation. In addition, the β-cell microcapsules can intelligently release insulin. The constructed living cell biosystem was further demonstrated its potential as artificial islets to be transplanted into diabetic mice omentum pouch to control blood glucose levels and thus treat diabetes of mice. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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