| Autor: |
Pastorek, Michal, Suchoňová, Mária, Konečná, Barbora, Pásztor, Slavomír, Petrus, Jakub, Ivašková, Nadja, Celec, Peter, Gardlík, Roman, Machala, Zdenko, Tóthová, Ľubomíra |
| Předmět: |
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| Zdroj: |
Plasma Chemistry & Plasma Processing; May2022, Vol. 42 Issue 3, p561-574, 14p |
| Abstrakt: |
Urinary tract infections (UTI) are often caused by resistant uropathogenic bacteria and can lead to sepsis or chronic renal failure. Cold plasma activated liquid (PAL) has known antimicrobial properties with applications in wound disinfection. The aim of our study is to evaluate PAL as a potential treatment of UTI in an animal model. Based on in vitro tests using a uropathogenic E. coli strain, PAL generated in the atmospheric air Glow Discharge plasma had the strongest antimicrobial effect in comparison to other types of PAL and was further tested in vivo. Transurethral PAL application had no effect on bacterial load in the 24 h mouse model of UTI. Upon investigating the treatment failure, we found that urine completely prevented any antimicrobial effects of PAL and PAL treatment of neutrophils resulted in their reduced viability and loss of mitochondrial membrane potential. These results do not support the hypothesis that the in vitro antimicrobial effects of PAL can be translated to the in vivo model of UTI. This could be explained by the attenuating effect of urine on the antimicrobial activity of PAL and its toxicity on immune cells. The detailed mechanism of the effects of urine on PAL requires further investigation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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